mouse monoclonal IgG1; IL-7 Antibody (D-9) is an IgG1 κ mouse monoclonal IL-7 antibody (also designated IL-7 antibody) that detects the IL-7 protein of human origin by WB, IP, IF, IHC(P) and ELISA. IL-7 Antibody (D-9) is available as both the non-conjugated anti-IL-7 antibody form, as well as multiple conjugated forms of anti-IL-7 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Interleukin-7 (IL-7) was originally described as a factor capable of inducing in vitro proliferation of pre-B cells from marrow cultures. The IL-7 gene encodes a protein 177 amino acids in length. IL-7 exerts its biological function through the IL-7 receptor which is expressed on pre-B cells, thymocytes and bone marrow-derived macrophages. The IL-7 receptor is composed of an IL-7 receptor-specific chain and the IL-2 receptor γ chain common to the IL-2, IL-4, IL-7, IL-9 and IL-15 receptors. IL-7 stimulation leads to the activation of Janus tyrosine kinase family members JAK1 and JAK3. Other studies have shown that in T cells, the IL-7 receptor-specific chain associates with the Src kinases family Lck and Fyn. IL-7 induces phosphorylation of insulin receptor substrate-1 (IRS-1) and insulin receptor substrate-2 (IRS-2), originally called 4PS.