High dose of biotin may cause interference with many routine clinical immunoassays and inaccurate results of some of the tests such as cardiac troponin has serious clinical complications. There is an increasing trend of high dose biotin supplement use in the US population, although the exact prevalence is still unknown. High dose of biotin supplement use may continue to increase due to its health and medical benefits and disrupt the diagnostic industry so some of the tests with serious clinical complications should be resigned to avoid biotin interference. Last but not least, more research is needed to understand the nutritional and therapeutic values of biotin.

Learning Objectives:

• Review mechanisms and factors that determine high dose biotin interference with lab tests
• Describe an increasing trend of high dose biotin use and health and medical benefits
• Explain why the continuing trend of high dose biotin use may be a game changer of the clinical diagnostic industry
• Make a case for why more research is needed to understand nutritional and therapeutic values of biotin