Recent advances in in vitro 3D cellular culture technologies, such as organoids, rapidly developed and established novel, more physiologically relevant models for basic biology and clinical applications. Traditionally, high-throughput microfluidics and other similar technologies have relied on two-dimensional (2D) cell models for the study of various human conditions and diseases, yet they are not compatible with 3D culture. I will discuss the development of an automatic and dynamic microfluidic ex vivo organoid culture modeling system to facilitate and accelerate preclinical research and the development of personalized treatment strategies. This integrated platform combines microfluidics, automatic dynamic and temporal stimulations (ie combinatorial drug screenings), combined with in silico data analysis and prediction tools. The platform can be used to advance the capabilities of research on cancer organoids models, screen and mirror real life patient chemotherapy treatments and ultimately facilitate treatment decisions for the development of personalized therapies.

 

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