Multiplexing IHC is a technique that is increasing in demand as more and more researchers and physicians are being tasked with doing more with less. With multiplexing, you have the advantage to view different targets and their levels of expression in relation to one another. In this presentation, we will review the different technologies and techniques that are currently available to assist with developing a multiplex assay. After a brief overview of the technologies, we will apply some of those technologies and walk through an example of optimizing a multiplex assay. Although digital analysis of multiplexed slides can be an important factor in the interpretation of a multiplexed assay, this presentation will focus on and emphasize the staining aspects of the assay optimization.

Learning Objectives: 

1. Understand the utility and methods for multiplexing
2. Understand the principles behind multiplex assay development