Field Applications Scientist, Cell Therapy, Thermo Fisher Scientific
BIOGRAPHY
Speaker
Abstract
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DATE: December 6, 2018
TIME: 9:00am PST, 12:00pm EST
Induced pluripotent stem cells (iPSC) intended for translational applications need to be of high quality; free of reprogramming transgenes, clear of adventitious agents, genetically stable, and functionally pluripotent. Generation of such cells requires consistent reprogramming, defined culture reagents and comprehensive characterization. CytoTune Sendai virus reprogramming enables consistent and robust reprogramming of diverse somatic cell types. Here we report the first off-the-shelf (CTS) Sendai virus reprogramming kit and a xeno-free workflow from fibroblast and blood cells designed for clinical and translational use. iPSCs were generated under these conditions from fibroblasts, CD34+ and T cells. To further streamline characterization, three molecular methods PluriTest, ScoreCard, and KaryoStat were used to confirm pluripotency, trilineage differentiation potential and genome integrity of the iPS cells.
Learning Objectives:
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The importance of consistent reprogramming, defined culture reagents and comprehensive characterization for generation of iPSC cells
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Details for the first off-the-shelf (CTS) Sendai virus reprogramming kit
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Review of three molecular methods PluriTest, ScoreCard, and KaryoStat and how they were used to confirm pluripotency
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