CRISPR/Cas gene editing has become the gold standard for individual gene perturbations as well as high-throughput functional screenings, with a rapidly increasing demand for high-quality CRISPR/Cas reagents requiring novel and innovative technologies to satisfy all user groups. The recently described 3Cs technology to generate bias-free gRNA and shRNA reagents addressees this issue and provides a robust and rapid, PCR- and cloning-free protocol for the generation of single and multiplexed gRNAs for functional interrogations. In my lecture, I will present selected single and multiplex gene editing applications to demonstrate the general applicability and power of 3Cs reagents.

Learning Objectives: 

1. What defines 3Cs gene editing reagents
2. What are the current forms of 3Cs reagents