Engineered nucleases like the CRISPR-Cas9 system provide researchers with powerful tools for the manipulation of the genome to create cell-based disease models, animal models, and cell therapies for clinical implementation. These editing modalities can achieve high editing efficiencies in a wide range of different cell types and gene loci. However, this high editing efficiency comes at a price: Off target cleavage. Growing concerns for off-target editing and the downstream results of such edits have researchers looking for best practices in their genome editing experiments. In this presentation, we will explore strategies to limit off-target edits in your gene editing experiments, share considerations to take in different experimental scenarios, and demonstrate the efforts of Thermo Fisher Scientific to optimize the gene editing workflow - simplifying it for all researchers.

 

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