The CSIRO Tissue Culture Facility (CTCF) employs a range of in-house optimized platform technologies to identify optimum vector-host cell combinations for both transient and stable protein production in traditional or single-use bioreactors. Our seminar will summarize the recommended steps to use these technologies and provide a quick guide to produce proteins in a cost-effective way. Also, a direct comparison of protein production using transient HEK 293 and the latest ExpiCHO cell cultures will be presented in detail.