A well-known problem of induced Pluripotent StemCell (iPSC) culture is the spontaneous acquisition of genetic abnormalities during culture. In order to check the health of our iPSC banks, we require an accurate and reliable QC solution to verify the health of our cells.

We have investigated multiple different techniques and services to assess the cytogenetic health of our cells, ranging from G banding through array-based PCR solutions to next generation technologies. We provide here a summary of our findings and why we have settled on the Bionano Saphyr as the best solution for cytogenetic QC.