This webinar will introduce why deep structural elucidation of drug candidate metabolism is a requisite part of early- to late-stage drug discovery. Early studies are used to determine if a drug candidate is prone to metabolic breakdown or oxidation/conjugation. This presentation showcases how both electron activated dissociation (EAD) and collision induced fragmentation (CID) bring new levels of interpretive power to the routine, but often challenging, process of metabolite identification. EAD utilizing a high-energy, electron-based fragmentation mechanism produces alternative fragments compared to CID. EAD generates a richer, more in depth fragmentation pattern than CID and can generate site specific spectral peaks for labile fragments that would otherwise be lost.

 

Learning Objectives