CRISPR based gene editing has proven to be an incredibly powerful tool for studying functions of individual proteins, understanding the role of multi-gene pathways, and even knocking out protein function genome-wide for drug candidate screening. With rapidly increasing amounts of information, reagent formats, and downstream applications, there is a lot to consider before beginning a CRISPR experiment.
Key questions to address include: What to consider when choosing a representative biological system? Which reagents are most suitable for your experimental aim? What techniques and equipment will be required for end-point data analysis?

In this webinar we will discuss:

• tips for choosing a cell line/type and the reagent formats best suited for that system
• methods for optimizing reagent delivery
• ways of enriching for edited cells
• techniques for analyzing post-edited cells

Learning objectives:

1. Identify key criteria necessary for determining the ideal cell line and reagent format for your gene editing experiment.

2. Outline techniques and equipment required for end-point data analysis