This is an introduction to the biological mechanism of RNA interference (RNAi) and experimental application of short, interfering RNA (siRNA). The key steps of the endogenous RNAi pathway will be explained to provide context for the mechanism of targeted gene knockdown with siRNA. The critical factors that affect siRNA function and specificity (off-targeting), such as siRNA design, chemical modifications and concentration, will be covered in detail. The practical application of siRNA will be covered through discussion of best-practice for experimental design. This will include proper use of positive and negative controls, optimization of transfection, and siRNA concentration. Measurement of gene knockdown and appropriate interpretation of experimental outcomes will round out the discussion. In this webinar, attendees will learn:

• Key steps of the endogenous RNAi pathway
• siRNA mechanism of action
• Best practices for siRNA experimental design
• Appropriate interpretation of experimental outcomes