In quantitative polymerase chain reaction (qPCR), multiplexing increases sample throughout, while reducing the time and cost associated with having separate reactions for each target. Furthermore, the variations that occurs between reactions are minimized when two targets of interest are measured within the same reaction. Due to these benefits, multiplexing in qPCR are widely used in different areas of research, with many applications such as diagnostics, treatment input and response monitoring, etc. While multiplexing in qPCR is advantageous, challenges such as assay design, workflow optimization, and reagent selection need to be addressed to help ensure the accuracy of the experimental output. In this webinar, the factors that impact multiplexing in qPCR are discussed and tools from Thermo Fisher Scientific that are designed for multiplexing success are presented including Applied Biosystems TaqMan Custom QSY and QSY2 based probes and TaqPath DuraPlex 1-Step RT-qPCR Master Mix.

Learning Objectives

1. Multiplexing for qPCR workflows

   • Benefits and condsiderations

   • Assay design guidelines

2. Thermo Fisher Scientific custom oligos for multiplexing needs

   • ​​​​​Applied Biosystem TaqMan Custom QSY and QSY2 based probes.

3. Features of master mixes designed for multiplexing.

   • Portfolio of master mixes with multiplexing capabilities