In the study of a mucosal vaccine for influenza, we need to evaluate the immune responses against vaccine in the serum and nasal washes. Serum IgG antibodies and mucosal secretory IgA antibodies are needed to evaluate. We use the mammalian protein expression system to produce the strain-specific influenza virus HA proteins to check the antibody titers. Moreover, we take the advantage of the expression system to produce the antibodies itself which were induced by the mucosal vaccine to characterize the effectiveness of the vaccine.