Targeted metabolic profiling enables confident measurements for known compounds of interest, yet to reproducibly measure concentration levels for these key metabolites, especially in large-scale applications, analytical rigor is required. While confidently identified, profiling select targets of the observed phenotype may not fully explain underlying biochemical mechanisms necessitating subsequent sample analyses. High-resolution accurate-mass Thermo Scientific™ Orbitrap™ technology enables unbiased compound detection for the accurate and quantitative analysis of target metabolites and the retro-mining of data to discover unknown biomarkers.