According to the National Forensics Laboratory Information System (NFLIS), the number of fentanyl-related reported cases increased from 945 cases in 2013 to 71,341 cases in 2017 while case load has risen minimally over this same time span.  Laboratories have established methods for known fentanyl analogs and other clinically relevant opioids, but the list of new illicit fentanyl analogs is rapidly expanding and can be challenging for laboratories to confirm without certified reference standards.  Here we present a method for fentanyl screening and semi-quantitation in urine for all 212 fentanyl analog compounds contained in the Centers for Disease Control (CDC) Fentanyl Analog Screening (FAS) Kit and Emergent Panels V1-V3 using HRAM mass spectrometry on the Thermo Scientific™ Orbitrap Exploris™ 120 mass spectrometer.