Over the past ten years, we’ve seen tremendous advances in 3D cell technologies, such as 3D in vitro models that more closely reflect morphological characteristics, cellular complexity, and physiology of tissues and organ systems in the human body. In this session, our speakers will discuss how the era of 3D biology is transforming preclinical research today. They will share how they are applying 3D liver models to investigate the toxic response to exposure to forever chemicals (per- and polyfluoroalkyl substances), introduce novel approaches for developing immune-competent 3D tumor models from fresh patient tumor tissue to better predict patient response to immunotherapies, identify synergies between translational and regulatory science in the context of microphysiological systems that mirror interactions between multiple tissue types, and provide expert advice on how to successfully implement these types of new 3D cell technologies in your workflow.