While chemiluminescence has been the traditional approach to western blot detection, this strategy is being progressively supplemented or replaced with fluorescence-based detection. With the introduction of advanced CCD-camera–based imaging systems and scanning instruments, along with improvements in fluorescent conjugate technologies, scientists now have the necessary tools available to take advantage of the range of fluorescent dyes and antibodies for fluorescent western blot detection. Learn more about best practices to help enable you to become successful at fluorescent western blotting.

Learning Objectives:

• Understand the fundamentals of fluorescent western blotting
• Successfully design fluorescent western blotting experiments