Genome engineering technologies have revolutionized genetic research and dramatically accelerated the pace of discovery. While knockout editing has garnered much of the attention, efforts are increasing to build new tools for creating knock-in cell models in both immortalized cell lines and primary cells, such as T-cells and iPSCs. This seminar will highlight recent advancements in gene editing tools, including application data, that demonstrate the power and ease of a new platform to design genome editing experiments and how innovative donor DNA strategies can be combined with next-generation CRISPR-Cas9 tools and TAL effector nucleases.