Discover the continued innovation in imaging and microscopy with Agilent's detailed application compendium on widefield microscopy for three-dimensional (3D) cell culture. This resource emphasizes the importance of 3D cell culture in replicating the natural cellular microenvironment, which is crucial for accurate biological research and drug testing. It also highlights the innovative techniques and tools that enhance the creation and analysis of 3D cell cultures, paving the way for more reliable and efficient scientific discoveries.
What you'll learn:
- Various imaging assays performed with Agilent BioTek high-content imaging instruments and Gen5 imaging software to characterize phenotypic changes in cancer spheroids in response to different compound treatments.
- Optimized spheroid cell culture protocols for common models, including low-adhesion U-bottom plates, Elplasia microwell plates, and the hanging drop method for three common cancer cell lines.
- An improved workflow for staining that reduces assay time and minimizes variability, using suitable reagents and liquid handling systems, along with a z-projection algorithm for efficient spheroid image acquisition and automated image analysis in Gen5.