Ritchie and Walker did much of the work that went into creating Dolly. They were working at the Roslin Institute on a project that aimed to create precise genetic alterations in farm animals. The research team leader, embryologist Ian Wilmut, thought that they could make those changes by altering the genome of a cell in culture. The nucleus of that cell, containing the changed DNA would then be transferred to a new cell. The scientists discuss the technical accuracy required for such delicate procedures – even a hangover could cause their hands to shake too much.
Photo from Reuters
Remarks Walker, “Tedious is absolutely the word. You're sitting, looking down a microscope and you've got both hands on the micromanipulators. It's kind of like the joysticks kids use nowadays on games. If your elbow slipped, you could wipe the whole dish out.”
The researchers have some nostalgia about their work, and return to the laboratories where Dolly was made. They also relate the hysteria, and the sheer volume of feedback that came along with the announcement of their discovery.
Ian Wilmut reminds us “Dolly is described as the first mammal cloned from an adult cell. She's actually the first adult clone, period. She's often undersold.”
Source: Nature