Assay developers in biopharma and research labs strive to save time, reduce costs, and ensure accurate and reproducible results. In quantitative PCR (qPCR) assays, multiplexing—the simultaneous detection of multiple targets per reaction with a single sample input—offers a solution to achieve these goals. However, multiplexing while maintaining accuracy, efficiency, and performance at scale-up poses significant hurdles.
In this application note, we explore the challenges faced by assay developers in multiplexing with qPCR, and we present data on reagents that enable assay developers to design their multiplex assay for up to six targets.