Welcome to For the Love of Digital PCR 2024 -
an immersive virtual event dedicated to exploring the remarkable capabilities of digital PCR. This experience invites you to delve into the forefront of scientific innovation across diverse research fields such as oncology research, environmental testing, cell and gene therapy and more.

Join us in celebrating the groundbreaking work of our esteemed customers, who are harnessing the power of the Applied Biosystems™ QuantStudio™ Absolute Q™ Digital PCR system to drive advancements and achievements in their respective fields. Through a curated program, this event offers a glimpse into the forefront of research and presents valuable insights to help propel your own work forward.


Sessions are open now!

In this third session we will explore:

CYP2D6 Copy Number Determination using Digital PCR
Speakers: Andrea Gaedigk, PhD and Wendy Wang

Accurate prediction of CYP2D6 activity from genotype data is of utmost importance as CYP2D6 pharmacogenetic (PGx) testing is increasingly used to guide drug therapy. However, given the complexity of the highly polymorphic CYP2D6 gene locus, PGx testing is not trivial and presents considerable challenges especially regarding copy number testing to capture the many structural variants. In this webinar, we will introduce a streamlined digital PCR method utilizing the Applied Biosystems™ QuantStudio™ Absolute Q™ dPCR System to detect CYP2D6 structural variation. This method integrates “one-pot” enzymatic restriction digestion with a custom triplex TaqMan™ copy number variation (CNV) assay, enabling the detection of three CYP2D6 regions in a single dPCR reaction. We will demonstrate how this approach enables accurate, reliable CNV quantification for CYP2D6 across various sample types including blood, saliva, and liver tissue. Join us to explore how this technique can help overcome common challenges in CYP2D6 evaluation, ultimately aiding in more accurate and effective drug therapy research and potential clinical testing.

For Research Use Only. Not for use in diagnostic procedures