Practical Considerations for Designing and Executing High-content Screens

High-content screening (HCS) is an imaging-based, multi-parametric strategy used in drug development that generates rich datasets through multiplexing strategically chosen fluorescent dyes and antibodies to visualize and measure biological phenomena. This is a powerful and flexible approach that can be used to answer diverse biological questions, from investigating the activation of a specific pathway to characterizing whole-cell responses to myriad treatments. This webinar will cover the basic principles and process of HCS with tips on how to design an informative and successful assay. To illustrate the power and versatility of HCS, we will include examples of specific use cases in various disease areas, including an overview of the technique of Cell Painting. These examples will feature a variety of in vitro models, from 2D to 3D cultures of single or multiple cell types. We will also review the benefits of working with a service provider, and what you can expect from working with PhenoVista. This webinar will discuss designing in vitro experiments to best answer your biological questions, how to prepare your test articles for high-content experiments, and how PhenoVista images and analyzes your experiments to yield valuable data. Thermo Fisher Scientific product manager, Steve Titus will also walk you through planning your cell painting experiment using the free web-based tool, Stain-iT Cell Staining Simulator.

 

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