mouse monoclonal IgG2b; Clathrin LC Antibody (CON.1) is an IgG2b κ mouse monoclonal Clathrin LC antibody (also designated Clathrin LC antibody) that detects the Clathrin LC protein of broad species origin by WB, IP and IF. Clathrin LC Antibody (CON.1) is available as both the non-conjugated anti-Clathrin LC antibody form, as well as multiple conjugated forms of anti-Clathrin LC antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Clathrin is a major cytosolic coat protein in pits and vesicles originating from the plasma membrane and the trans-Golgi network. In receptor-mediated endocytosis, receptor proteins are engulfed by clathrin-coated vesicles. Clathrin is composed of three heavy chains and three light chains which associate non-covalently to form a triskelion structure. Clathrin light chain regulates the self-assembly of triskelions onto intracellular membranes. Clathrin light chain subunits (LCA and LCB) contribute to regulation of coated vesicle formation to sort proteins during receptor-mediated endocytosis and organelle biogenesis. Although LCA and LCB are encoded by two discrete genes sharing only 60% homology, they have certain features in common. Both LCA and LCB undergo alternative mRNA splicing, which results in the generation of tissue-specific isoforms. Additionally, in the brain, LCA and LCB contain inserted sequences that form higher molecular weight isoforms. These sequences insert at similar cytoplasmic domain encoding regions for both LCA and LCB.