mouse monoclonal IgG2a; CUL-1 Antibody (D-5) is an IgG2a κ mouse monoclonal CUL-1 antibody (also designated Cullin-1 antibody or CUL1 antibody) that detects the CUL-1 protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. CUL-1 Antibody (D-5) is available as both the non-conjugated anti-CUL-1 antibody form, as well as multiple conjugated forms of anti-CUL-1 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Cullin proteins comprise a distinct family of mediators that participate in the selective targeting of proteins for ubiquitin (Ub)-mediated proteolysis. CUL-1, which is the mammalian homolog of yeast Cdc53, is an integral component of the E3 ubiquitin ligase complex designated SCF. The SCF (Skp1/CUL-1/F-box protein complex) consists of Skp1 associating with both CUL-1 and an F-box protein, such as Skp2, which determines the substrate specificity of the complex. CUL-1-mediated ubiquitination results in the degradation of cell cycle proteins cyclin D, p21 and cyclin E. Another cullin, CUL-3, facilitates the degradation of cyclin E independent of SCF activity, while CUL-2 associates with the tumor suppressing protein VHL and elongin B to form VBC complexes, which structurally resemble the SCF ligase. Proteolysis also occurs by way of CUL-4 associating with Nedd-8, a ubiquitin-like protein, where it too functions as an active component of a multifunctional E3 complex. CUL-5, also designated vasopressin-activated, calcium-mobilizing protein (VACM-1), is also included in the cullin family as it shares substantial sequence homology with CUL-1.