mouse monoclonal IgG1; karyopherin β2/2B Antibody (A-11) is an IgG1 κ mouse monoclonal karyopherin beta 2/2B antibody (also designated Transportin 1/2 antibody) that detects the karyopherin beta 2/2B protein of mouse, rat and human origin by WB, IP, IF and ELISA. karyopherin β2/2B Antibody (A-11) is available as both the non-conjugated anti-karyopherin beta 2/2B antibody form, as well as multiple conjugated forms of anti-karyopherin beta 2/2B antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Protein transport across the nucleus is a selective, multi-step process involving several cytoplasmic factors. Proteins must be recognized as import substrates, dock at the nuclear pore complex and translocate across the nuclear envelope in an ATP-dependent fashion. Two cytosolic factors centrally involved in the recognition and docking process are the karyopherin α1 and karyopherin β1 subunits. Karyopherin α1 functions in the recognition and targeting of substrates destined for nuclear import, while karyopherin β1 serves as an adapter, tethering the karyopherin α1/substrate complex to docking proteins on the nuclear envelope, termed nucleoporins. Karyopherin α2 has been shown to complex with Epstein-Barr virus nuclear antigen 1 (EBNA-1). Karyopherin β2 and karyopherin β2B (also designated transportin 1 and transportin 2) share 84% sequence identity at the amino acid level, however, they have been shown to have different substrate specificities. Karyopherin β2 mediates hnRNPA1 nuclear import while karyopherin β2B has been implicated in the export of cellular mRNAs through complexes formed with the mRNA export factor TAP.