mouse monoclonal IgG2a; LIME Antibody (A-7) is an IgG2a κ mouse monoclonal LIME antibody (also designated LIME antibody) that detects the LIME protein of mouse, rat and human origin by WB, IP, IF and ELISA. LIME Antibody (A-7) is available as both the non-conjugated anti-LIME antibody form, as well as multiple conjugated forms of anti-LIME antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Lck-interacting molecule (LIME) is a 295 amino acid transmembrane adaptor protein primarily expressed in hematopoietic and lung cells. LIME has a short extracellular domain and a cytoplasmic tail containing five tyrosine-based motifs. LIME becomes tyrosine-phosphorylated after the CD4 or CD8 co-receptors cross-link. The phosphorylated LIME interacts with Lck, the Src fam-ily kinase, and Csk, its negative regulator. LIME is expressed during the early and late stages of T cell activation and appears to be involved in regulation of T cell activation by co-receptors. It may be involved in activation of the ERK and JNK (both are part of the mitogen-activated protein kinase family) pathways in T cells. BCR-mediated B cell activation may also involve LIME.