mouse monoclonal IgG1; Sp1 Antibody (1C6) is an IgG1 κ mouse monoclonal Sp1 antibody (also designated Sp1 transcription factor antibody) that detects the Sp1 protein of mouse, rat and human origin by WB, IF, IHC(P) and ELISA. Sp1 Antibody (1C6) is available as both the non-conjugated anti-Sp1 antibody form, as well as multiple conjugated forms of anti-Sp1 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Sp1 is a sequence-specific transcription factor that recognizes GGGGCGGGGC and closely related sequences, which are often referred to as GC boxes. Sp1 was initially identified as a HeLa cell-derived factor that selectively activates in vitro transcription from the SV40 promoter and binds to the multiple GC boxes in the 21-bp repeated elements in SV40. The sequence specificity of DNA binding is conferred by Zn (II) fingers, whereas a different region of Sp1 appears to regulate the affinity of DNA binding. Sp1 belongs to a subgroup of transcription factors that are phosphorylated upon binding to promoter sequences. Evidence suggests that the early growth response gene, Erg-1 (also known as Zif268 or NGF1-A), may downregulate certain mammalian gene promoters by competing with Sp1 for binding to an overlapping binding motif. The gene encoding human Sp1 maps to chromosome 12q13.1.