mouse monoclonal IgG2a; Nlk Antibody (B-5) is an IgG2a mouse monoclonal Nlk antibody (also designated Serine/Threonine-Protein Kinase NLK antibody, Nemo-Like Kinase antibody, Protein LAK1 or LAK1 antibody) that detects the Nlk protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. Nlk Antibody (B-5) is available as both the non-conjugated anti-Nlk antibody form, as well as multiple conjugated forms of anti-Nlk antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2 (1-4), respectively. ERK proteins are regulated by dual phosphorylation at specific tyrosine and threonine sites mapping within a characteristic Thr-Glu-Tyr motif. Phosphorylation at both Thr 183 and Tyr 185 is required for full enzymatic activation. In response to activation, MAP kinases phosphorylate downstream components on serine and threonine. Nlk, or nemo-like kinase, is a murine homolog of the Drosophila nemo (nmo) gene. Nlk and Nmo have sequence homology to both the ERK MAP kinases and the cyclin dependent kinases. Nlk is a nuclear protein with the ability to autophosphorylate.