mouse monoclonal IgG2a; Ku70 Antibody (A-9) is an IgG2a κ mouse monoclonal Ku70 antibody that detects Ku70 of human origin by WB, IP, IF, IHC(P), FCM and ELISA. Ku70 Antibody (A-9) is available as the non-conjugated anti-Ku70 antibody form, as well as multiple conjugated forms of anti-Ku70 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. The Ku protein is localized in the nucleus and is composed of subunits referred to as Ku70 (p70) and Ku86 (p86) which is also known by the synonym Ku80 or (p80). Ku was first described as an autoantigen to which antibodies were produced in a patient with scleroderma polymyositis overlap syndrome, and was later found in the sera of patients with other rheumatic diseases. Both subunits of the Ku protein have been cloned, and a number of functions have been proposed for Ku, including cell signaling, DNA replication and transcriptional activation. Ku is involved in Pol II-directed transcription by virtue of its DNA binding activity, serving as the regulatory component of the DNA-associated protein kinase that phosphorylates Pol II and transcription factor Sp. Ku proteins also activate transcription from the U1 small nuclear RNA and the human transferrin receptor gene promoters. A Ku-related protein designated the enhancer 1 binding factor (E1BF), composed of two subunits, has been identified as a positive regulator of RNA polymerase I transcription initiation.