mouse monoclonal IgG1; Vimentin Antibody (V9) is an IgG1 κ mouse monoclonal Vimentin antibody (also designated VIM antibody) that detects the Vimentin protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM. Vimentin Antibody (V9) is available as both the non-conjugated anti-Vimentin antibody form, as well as multiple conjugated forms of anti-Vimentin antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. Intermediate filaments are constructed from two-chain, a-helical, coiled-coil molecules arranged on an imperfect helical lattice and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. One such intermediate filament protein, Vimentin, is a general marker of cells originating in the mesenchyme. Vimentin is frequently coexpressed with other members of the intermediate filament family, such as the cytokeratins, in neoplasms including melanoma and breast carcinoma. Showing broad mammalian species reactivity, the Vimentin Antibody (V9) displays both cytoplasmic and membrane localization in immunostaining applications.