mouse monoclonal IgG1; PPARγ Antibody (E-8) is an IgG1 κ mouse monoclonal PPAR gamma antibody (also designated PPARG antibody) that detects the PPAR gamma protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. PPARγ Antibody (E-8) is available as both the non-conjugated anti-PPAR gamma antibody form, as well as multiple conjugated forms of anti-PPAR gamma antibody, including agarose, PE, FITC and multiple Alexa Fluor® conjugates. PPARs are members of the nuclear hormone receptor subfamily of transcription factors. PPARs form heterodimers with retinoid X receptors (RXRs). These heterodimers regulate transcription of genes involved in insulin action, adipocyte differentiation, lipid metabolism and inflammation. PPARγ is implicated in numerous diseases including obesity, diabetes, atherosclerosis and cancer, making the PPARγ antibody an important research tool. PPARγ activators include prostanoids, fatty acids, thiazolidinediones and N-(2-benzoylphenyl) tyrosine analogues. A key component in adipocyte differentiation and fat-specific gene expression, PPARγ may modulate macrophage functions such as proinflammatory activities, and stimulate oxidized low-density lipoprotein (x-LDL) uptake. A Pro12Ala polymorphism of the PPARγ2 gene has been reported to reduce transactivation activity in vitro. This substitution may affect the immune response to ox-LDL and be associated with type 2 diabetes. In addition, the Pro12Ala variant of the PPARγ2 gene maybe correlated with abdominal obesity in type 2 diabetes. The PPARγ Antibody (E-8) is recommended for detection of both PPARγ1 and PPARγ2 gene products.