mouse monoclonal IgG2a; Cdk7 Antibody (C-4) is an IgG2a κ mouse monoclonal Cdk7 antibody (also designated CDKN7 antibody, p39 Mo15 antibody, CAK antibody or Cyclin-dependent kinase 7 antibody) that detects the Cdk7 protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. Cdk7 Antibody (C-4) is available as both the non-conjugated anti-Cdk7 antibody form, as well as multiple conjugated forms of anti-Cdk7 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Progression through the cell cycle requires activation of a series of enzymes designated cyclin dependent kinases (Cdks). The monomeric catalytic subunit Cdk2, a critical enzyme for initiation of cell cycle progression, is completely inactive. Partial activation is achieved by the binding of regulatory cyclins such as cyclin D1, while full activation requires additional phosphorylation at Thr 160. The enzyme responsible for the phosphorylation of Cdk2 on Thr 160 and also of Cdc2 p34 on Thr 161, designated Cdk-activating kinase (CAK), has been partially purified and shown to be comprised of a catalytic subunit and a regulatory subunit. The catalytic subunit, designated Cdk7, has been identified as the mammalian homolog of MO15, a protein kinase demonstrated in starfish and Xenopus. The regulatory subunit is a novel cyclin (cyclin H) and is required for activation of Cdk7. Like other Cdks, Cdk7 contains a conserved threonine residue required for full activity; mutation of this residue severely reduces CAK activity.