mouse monoclonal IgG1; Bcl-2 Antibody (C-2) is an IgG1 κ mouse monoclonal Bcl-2 antibody (also designated BCL2 antibody or PPP1R50 antibody) that detects the Bcl-2 protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM. Bcl-2 Antibody (C-2) is available as both the non-conjugated anti-Bcl-2 antibody form, as well as multiple conjugated forms of anti-Bcl-2 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Bcl-2 is one among many key regulators of apoptosis, which are essential for proper development, tissue homeostasis, and protection against foreign pathogens. Immunostaining of human tissues using the Bcl-2 antibody shows cytoplasmic and membrane staining, as human Bcl-2 is an anti-apoptotic, membrane-associated oncoprotein. The Bcl-2 protein promotes cell survival through protein-protein interactions with other Bcl-2 related family members, such as the death suppressors Bcl-xL, Mcl-1, Bcl-w, and A1 or the death agonists Bax, Bak, Bik, Bad, and Bid. The anti-apoptotic function of Bcl-2 can also be regulated through proteolytic processing and phosphorylation. Bcl-2 may promote cell survival by interfering with the activation of the cytochrome c/Apaf-1 pathway through stabilization of the mitochondrial membrane. Mutations in the Bcl-2 gene can contribute to cancers where normal physiological cell death mechanisms are compromised by deregulation of the anti-apoptotic influence of Bcl-2.