mouse monoclonal IgG1; CIS Antibody (D-8) is an IgG1 κ mouse monoclonal CIS antibody (also designated CIS antibody) that detects the CIS protein of human origin by WB, IP, IF, IHC(P) and ELISA. CIS Antibody (D-8) is available as both the non-conjugated anti-CIS antibody form, as well as multiple conjugated forms of anti-CIS antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Src homology (SH2) domains are noncatalytic sequences that are conserved among a number of cytoplasmic signaling proteins. These signaling proteins are directly regulated by receptor tyrosine kinases and control the activation of mitogenic signal transduction pathways by such receptors. For instance, ligand-induced activation of the EGF and PDGF receptors induces dimerization, triggers receptor autophosphorylation on tyrosine residues and results in the binding of a number of cytoplasmic SH2 domain proteins such as PLC-g1, Ras GAP and PI 3 kinase p85 to the activated receptors. The Shc gene encodes three proteins with a single SH2 domain but no identifiable catalytic domain. CIS, cytokine-inducible SH2-containing protein, is a 267 amino acid protein with a single 96 amino acid SH2 domain that associates the tyrosine-phosphorylated beta chain of the IL-3 receptor with the tyrosine-phosphorylated Epo receptor. CIS was initially described as an immediate early cytokine-responsive gene and appears to be a unique regulator of cytokine signaling.