mouse monoclonal IgG2a; VP16 Antibody (14-5) is an IgG2a κ mouse monoclonal VP16 antibody (also designated VP16 antibody or Herpes simplex virus protein vmw65 antibody) that detects VP16 and VP16 fusion proteins by WB, IP and IF. VP16 Antibody (14-5) is available as the non-conjugated anti-VP16 antibody form, as well as multiple conjugated forms of anti-VP16 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. The GAL4 protein of Saccharomyces cerevisiae is one of the most thoroughly characterized transcriptional activators. Since the N-terminal 147 amino acid residues of GAL4 are sufficient to mediate specific and strong binding to DNA, but are incapable of efficient transcriptional activation, this protein fragment has frequently been used to confer specific DNA binding in experiments examining transcriptional activation functions of heterologous proteins. This approach is facilitated by the finding that higher eukaryotes lack endogenous proteins that enhance transcription from the consensus GAL4-binding site. Fusions between GAL4 (aa 1-147) and activating domains from a variety of transcriptional regulatory proteins can activate transcription in yeast, plant, insects and mammalian cells. A unique "two-hybrid" system has been developed using GAL4 fusions in yeast to identify specific protein-protein interactions. Another "two-hybrid" system utilizes the DNA binding domain of the E. coli protein Lex A and the transactivity domain of the HSV protein VP16.