mouse monoclonal IgG2a; IKKβ Antibody (H-4) is an IgG2a κ mouse monoclonal IKK beta antibody (also designated IKK beta antibody, IKBKB antibody, IKK2 antibody or IKKB antibody) that detects the IKK beta protein of human origin by WB, IP, IF, IHC(P), FCM and ELISA. IKKβ Antibody (H-4) is available as both the non-conjugated anti-IKK beta antibody form, as well as multiple conjugated forms of anti-IKK beta antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. The transcription factor NFkB is retained in the cytoplasm in an inactive form by the inhibitory protein IkB. Activation of NFkB requires that IkB be phosphorylated on specific serine residues, which results in targeted degradation of IkB. IkB kinase α (IKKα), previously designated CHUK, interacts with IkB-α and specifically phosphorylates IκBα on Serine 32 and 36, the sites that trigger its degradation. IKKα appears to be critical for NFkB activation in response to proinflammatory cytokines. Phosphorylation of IkB by IKKα is stimulated by the NFkB inducing kinase (NIK), which itself is a central regulator for NFkB activation in response to TNF and IL-1. The functional IKK complex contains three subunits, IKKα, IKKβ and IKKγ (also designated NEMO), and each appear to make essential contributions to IkB phosphorylation.