mouse monoclonal IgG2a; Histone H1 Antibody (AE-4) is an IgG2a κ mouse monoclonal Histone H1 antibody that detects Histone H1 of broad species origin by WB, IP, IF, IHC(P), FCM and ELISA. Histone H1 Antibody (AE-4) is available as the non-conjugated anti-Histone H1 antibody form, as well as multiple conjugated forms of anti-Histone H1 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.