mouse monoclonal IgG1; C23 Antibody (MS-3) is an IgG1 κ mouse monoclonal C23 antibody (also designated Nucleolin antibody or NCL antibody) that detects the C23 protein of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA. C23 Antibody (MS-3) is available as both the non-conjugated anti-C23 antibody form, as well as multiple conjugated forms of anti-C23 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. C23 (nucleolin, NCL) is a eukaryotic nucleolar phosphoprotein that influences synthesis and maturation of ribosomes. C23 localizes to dense fibrillar regions of the nucleolus. It contains four RNA binding domains that interact with pre-rRNA during synthesis. C23 can influence RNA processing, ribosomal gene transcription and nucleolar targeting of ribosomal components. It is known to associate with a variety of proteins, including the nucleolar protein B23. Phosphorylation by Cdc2 and casein kinase II causes translocation of C23 from the nucleolus to the cytoplasm. Mitotic phosphorylated forms of Bcl-2 are present in nuclear structures in prophase Hela cells together with C23 and Ki-67. Retinoic acid-induced apoptosis leads to C23 down-regulation and Bcl-2 mRNA instability. C23 binds the human telomerase reverse transcriptase subunit (TERT) through interactions with its RNA binding domain 4 and carboxyl-terminal RGG domain, and this interaction is critical for the nucleolar localization of human TERT.