mouse monoclonal IgG1; p-Trk Antibody (E-6) is an IgG1 κ mouse monoclonal p-Trk antibody (also designated p-Trk antibody or Pan Trk antibody) that detects Trk A phosphorylated at Tyr 496 and correspondingly phosphorylated Trk B and Trk C of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. p-Trk Antibody (E-6) is available as the non-conjugated anti-p-Trk antibody form, as well as multiple conjugated forms of anti-p-Trk antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. The Trk proto-oncogene encodes a membrane-spanning protein tyrosine kinase, Trk gp140, also designated Trk A, whose expression is restricted in vivo to neurons of the sensory spinal and cranial ganglia of neural crest origin. Nerve growth factor (NGF) stimulates tyrosine phosphorylation of Trk A in neural cell lines and in embryonic dorsal root ganglia. Tyrosine phosphorylation of Trk by NGF is rapid, specific and occurs with picomolar quantities of factor, indicating that the response is mediated by physiological amounts of NGF. Unlike Trk A, tyrosine phosphorylation of Trk B is induced by the brain-derived neurotrophic factor (BDNF) and, to a lesser extent, neurotrophin-3 (NT-3). Additionally, Trk C phosphorylation is induced via interaction with endogenously produced NT-3 and is not dependent on stimulation by NGF.