mouse monoclonal IgG1; PKC α Antibody (H-7) is an IgG1 κ mouse monoclonal PKC alpha antibody (also designated PRKCA antibody) that detects the PKC alpha protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. PKC α Antibody (H-7) is available as both the non-conjugated anti-PKC alpha antibody form, as well as multiple conjugated forms of anti-PKC alpha antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor-promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into many different isoforms (α, βI, βII, γ, δ, ε, ζ, η, θ, λ/ι, µ and ν). Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of PKC δ and ε are independent of Ca2+. On the other hand, most of the other PKC members possess phorbol ester-binding activities and kinase activities.