mouse monoclonal IgG2a; Chk1 Antibody (G-4) is an IgG2a κ mouse monoclonal Chk1 antibody (also designated CHEK1 antibody, or checkpoint kinase 1 antibody) that detects the Chek1 gene product of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA. Chk1 Antibody (G-4) is available as both the non-conjugated anti-Chk1 antibody form, as well as multiple conjugated forms of anti-Chk1 antibody, including agarose, PE, FITC and multiple Alexa Fluor® conjugates. Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by proteolysis of cyclins. Chk1 and Chk2 are involved in these processes as regulators of Cdks. Chk1 and Chk2 both function as essential components in the G2 DNA damage checkpoint by phosphorylating Cdc25C in response to DNA damage. Phosphorylation inhibits Cdc25C activity, thereby blocking mitosis. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. It has also been shown that Chk1 can phosphorylate Wee1 in vitro, providing evidence that the hyperphosphorylated form of Wee1, seen in cells delayed by Chk1 overexpression, is due to phosphorylation by Chk1.