mouse monoclonal IgG1; RasGRP Antibody (199) is an IgG1 κ mouse monoclonal RasGRP antibody (also designated RasGRP antibody) that detects the RasGRP protein of mouse, rat and human origin by WB, IP, IF and ELISA. RasGRP Antibody (199) is available as both the non-conjugated anti-RasGRP antibody form, as well as multiple conjugated forms of anti-RasGRP antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. The superfamily of GTP-binding proteins, of which Ras proteins are prototypes, has been implicated in a broad range of biological activities. Studies have identified a family of guanine nucleotide-releasing factors (GRFs) that activate Ras in mammalian cells and an "adapter" protein (Sem 5/GRB2) that appears to mediate the interaction of GRFs with activated receptor molecules. Subsequent to activation, Ras appears to interact with Raf, thereby activating the MAP kinase phosphorylation pathway. RasGRP is a guanyl nucleotide-releasing protein for Ras that contains two EF hand domains, which bind to calcium, and a diacylglycerol (DAG)-binding domain. RasGRP is expressed in the nervous system and lymphoid tissues and may link changes in DAG and calcium concentrations to Ras activation.