mouse monoclonal IgG2b; TNF-R1 Antibody (H-5) is an IgG2b κ mouse monoclonal TNF-R1 antibody (also designated TNFR1 antibody, TNFRSF1A antibody, TNF receptor superfamily member 1A antibody) that detects the TNF-R1 protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. TNF-R1 Antibody (H-5) is available as both the non-conjugated anti-TNF-R1 antibody form, as well as multiple conjugated forms of anti-TNF-R1 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Tumor necrosis factor (TNF) is a pleiotropic cytokine whose function is mediated through two distinct cell surface receptors. These receptors, designated TNF-R1 and TNF-R2, are expressed on most cell types. The majority of TNF functions are primarily mediated through TNF-R1, while signaling through TNF-R2 occurs less extensively and is confined to cells of the immune system. Both of these proteins belong to the growing TNF and nerve growth factor (NGF) receptor superfamily, which includes FAS, CD30, CD27 and CD40. The members of this superfamily are type I membrane proteins that share sequence homology confined to the extracellular region. TNF-R1 shares a motif coined the "death domain" with FAS and three structurally unrelated signaling proteins, TRADD, FADD and RIP (1,3-8). This "death domain" is required for transduction of the apoptotic signal.