mouse monoclonal IgG2b; PDGF-A Antibody (E-10) is an IgG2b κ mouse monoclonal PDGF-A antibody (also designated Platelet-derived growth factor subunit A antibody, PDGF-1 antibody, or PDGFA antibody) that detects the PDGF-A protein of mouse, rat and human origin by WB, IP, IF and IHC(P). PDGF-A Antibody (E-10) is available as both the non-conjugated anti-PDGF-A antibody form, as well as multiple conjugated forms of anti-PDGF-A antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. PDGF is a mitogen for mesenchyme- and glia-derived cells. It consists of two disulfide-bonded polypeptide chains, A and B, and occurs as three isoforms, PDGF AA, PDGF AB, and PDGF BB. The three isoforms bind with different affinities to two receptor types, α and β, which are structurally related and endowed with protein-tyrosine kinase domains. Ligand binding induces activation of the receptor kinases by formation of receptor dimers; the A subunit of PDGF binds only to α receptors with high affinity, whereas the B subunit can bind to both α and β receptors. Evidence suggests that PDGF may function as a neurotrophic factor. The fact that receptors for DGF-A are expressed in oligodendrocyte progenitor cells whereas receptors for PDGF-B are expressed on neurons suggests that the different isoforms of PDGF may regulate growth and differentiation of different cell types in the developing central nervous system by paracrine and autocrine routes.