mouse monoclonal IgG1; eIF4E Antibody (P-2) is an IgG1 κ mouse monoclonal eIF4E antibody (also designated EIF4E antibody, or EIF4E1 antibody) that detects the eIF4E protein of mouse, rat, human and porcine origin by WB, IP, IF, IHC(P) and ELISA. eIF4E Antibody (P-2) is available as both the non-conjugated anti-eIF4E antibody form, as well as multiple conjugated forms of anti-eIF4E antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. The eukaryotic initiation complex eIF4F exists in vitro as a trimeric complex of eIF4E, eIF4A and eIF4G. Together, the complex allows ribosome binding to mRNA by inducing the unwinding of mRNA secondary structures. eIF4E binds to the mRNA "cap" during an early step in the initiation of protein synthesis. eIF4A acts as an ATP-dependent RNA helicase. eIF4G acts as a bridge between eIF4E, eIF4A and the eIF3 complex.