CRISPR is an abbreviation of Clustered Regularly Interspaced Short Palindromic Repeats. The name was minted at a time when the origin and use of the interspacing subsequences were not known. At that time the CRISPRs were described as segments of prokaryotic DNA containing short, repetitive base sequences. In a palindromic repeat, the sequence of nucleotides is the same in both directions. Each repetition is followed by short segments of spacer DNA from previous exposures to foreign DNA (e.g., a virus or plasmid). Small clusters of cas (CRISPR-associated) genes are located next to CRISPR sequences.
Stem cells can self-renew and differentiate into multiple different cell types. Engineering of stem cells has enabled new methods to study development and organogenesis in humans as well as d...
I will present my lab’s effort on studying and manipulating RNA processing, with particular emphasis on using CRISPR/Cas systems for targeting RNA in living cells in diseases such as my...
The manipulation of the genome to study disease, evaluate drug targets and to stratify patient populations is now a widely accepted concept in the field. At Horizon Discovery, genome engineer...
Prokaryotes have long been at war with bacteriophage. The evolutionary pressure of this struggle has led both sides to develop sophisticated defenses. Notably, CRISPR-Cas systems evolved to l...
DATE: August 22, 2017TIME: 9:00am PT, 12:00pm ETThe use of CRISPR-Cas9 to create targeted double-strand breaks in genomic DNA has greatly simplified strategies for precise gen...
EVENT DETAILS:DATE: July 25, 2017TIME: 9:00am PT, Noon ETThermo Fisher Scientific is proud to present the SyncD3 webinar series. As a thought-leader in science...
DATE: July 21, 2017TIME: 10:00am PT, 1:00pm ETCRISPR/Cas9 techniques are quickly growing in popularity for the generation of new mouse models for research. Though the approach is relati...
CRISPR-Cas9 systems provide a platform for high efficiency genome editing that are enabling innovative applications of mammalian cell engineering. The delivery of Cas9 plasmid DNA or mR...
The development of the CRISPR/Cas9 gene-editing platform enables the rapid generation of new genetically modified stem cell models of human diseases, as well as providing new potential therap...
EVENT DETAILS: DATE: June 13, 2017TIME: 9:00am PT, 12:00pm ETThermo Fisher Scientific is proud to present the SyncD3 webinar series. As a thought-leader in...
The emergence of technology for development of induced pluripotent stem cells (iPSCs) from somatic cells, such as skin and blood cells, has resulted in the ability of researchers to have limi...
EVENT DETAILS:DATE: May 16, 2017TIME: 9:00am PT, Noon ETThermo Fisher Scientific is proud to present the SyncD3 webinar series. As a thought-leader in science our first commit...
Do you have targets that are difficult to genotype? Are you starting new projects and looking for a fast convenient genotyping solution?Whether screening CRISPR clones, exploring pharmaco...
Developing therapies for human diseases continues to face obstacles, particularly in translating targets or compounds identified by in vitro screening campaigns to valid targets or efficaciou...
CRISPR-Cas9 has increased the accessibility of genome engineering due to its ease of use and ability to cause double strand breaks (DSBs) at almost any locus of interest. DSBs are repaired in...
There is rapidly growing interest in using the CRISPR-Cas9 system for functional screening, both as a primary screening tool and as an orthogonal tool for RNAi hit validation. High throughput...
The use of pluripotent stem cell-derived cell types for disease modeling, drug screening and regenerative medicine is an exciting area of activity in health research. Prior to the availabilit...
The CRISPR-Cas9 system is being widely used for genome engineering in many different biological applications. It was originally adapted from the bacterial Type II CRISPR system and uses a Cas...
This is an introduction to the biological mechanism of RNA interference (RNAi) and experimental application of short, interfering RNA (siRNA). The key steps of the endogenous RNAi pathway wil...
DATE: February 8, 2017TIME: 1:30pm PT, 4:30pm ETThe powerful and expanding use of genetically modified mice introduces important aspects of genetics in managing these strains for breedi...
DATE: December 13, 2016TIME: 8:00 AM PT, 11:00 AM ETUnderstanding physiological mechanisms regulating growth and nutrient deposition in rainbow trout is central to establish production...
The recent Zika virus outbreak highlights the need for low-cost diagnostics that can be rapidly developed for distribution and use in pandemic regions. In early 2016 we developed a pipe...
Over the last 15 years, human pluripotent stem cell (hPSC) technologies have progressed from academic curiosities into tools with the promise to underpin commerce, leading to real progress in...
The isolation of human embryonic stem cells (hESCs) and the discovery of human induced pluripotent stem cell (hiPSC) reprogramming have sparked a renaissance in stem cell biology, in vitro di...
Stem cells can self-renew and differentiate into multiple different cell types. Engineering of stem cells has enabled new methods to study development and organogenesis in humans as well as d...
I will present my lab’s effort on studying and manipulating RNA processing, with particular emphasis on using CRISPR/Cas systems for targeting RNA in living cells in diseases such as my...
The manipulation of the genome to study disease, evaluate drug targets and to stratify patient populations is now a widely accepted concept in the field. At Horizon Discovery, genome engineer...
Prokaryotes have long been at war with bacteriophage. The evolutionary pressure of this struggle has led both sides to develop sophisticated defenses. Notably, CRISPR-Cas systems evolved to l...
DATE: August 22, 2017TIME: 9:00am PT, 12:00pm ETThe use of CRISPR-Cas9 to create targeted double-strand breaks in genomic DNA has greatly simplified strategies for precise gen...
EVENT DETAILS:DATE: July 25, 2017TIME: 9:00am PT, Noon ETThermo Fisher Scientific is proud to present the SyncD3 webinar series. As a thought-leader in science...
DATE: July 21, 2017TIME: 10:00am PT, 1:00pm ETCRISPR/Cas9 techniques are quickly growing in popularity for the generation of new mouse models for research. Though the approach is relati...
CRISPR-Cas9 systems provide a platform for high efficiency genome editing that are enabling innovative applications of mammalian cell engineering. The delivery of Cas9 plasmid DNA or mR...
The development of the CRISPR/Cas9 gene-editing platform enables the rapid generation of new genetically modified stem cell models of human diseases, as well as providing new potential therap...
EVENT DETAILS: DATE: June 13, 2017TIME: 9:00am PT, 12:00pm ETThermo Fisher Scientific is proud to present the SyncD3 webinar series. As a thought-leader in...
The emergence of technology for development of induced pluripotent stem cells (iPSCs) from somatic cells, such as skin and blood cells, has resulted in the ability of researchers to have limi...
EVENT DETAILS:DATE: May 16, 2017TIME: 9:00am PT, Noon ETThermo Fisher Scientific is proud to present the SyncD3 webinar series. As a thought-leader in science our first commit...
Do you have targets that are difficult to genotype? Are you starting new projects and looking for a fast convenient genotyping solution?Whether screening CRISPR clones, exploring pharmaco...
Developing therapies for human diseases continues to face obstacles, particularly in translating targets or compounds identified by in vitro screening campaigns to valid targets or efficaciou...
CRISPR-Cas9 has increased the accessibility of genome engineering due to its ease of use and ability to cause double strand breaks (DSBs) at almost any locus of interest. DSBs are repaired in...
There is rapidly growing interest in using the CRISPR-Cas9 system for functional screening, both as a primary screening tool and as an orthogonal tool for RNAi hit validation. High throughput...
The use of pluripotent stem cell-derived cell types for disease modeling, drug screening and regenerative medicine is an exciting area of activity in health research. Prior to the availabilit...
The CRISPR-Cas9 system is being widely used for genome engineering in many different biological applications. It was originally adapted from the bacterial Type II CRISPR system and uses a Cas...
This is an introduction to the biological mechanism of RNA interference (RNAi) and experimental application of short, interfering RNA (siRNA). The key steps of the endogenous RNAi pathway wil...
DATE: February 8, 2017TIME: 1:30pm PT, 4:30pm ETThe powerful and expanding use of genetically modified mice introduces important aspects of genetics in managing these strains for breedi...
DATE: December 13, 2016TIME: 8:00 AM PT, 11:00 AM ETUnderstanding physiological mechanisms regulating growth and nutrient deposition in rainbow trout is central to establish production...
The recent Zika virus outbreak highlights the need for low-cost diagnostics that can be rapidly developed for distribution and use in pandemic regions. In early 2016 we developed a pipe...
Over the last 15 years, human pluripotent stem cell (hPSC) technologies have progressed from academic curiosities into tools with the promise to underpin commerce, leading to real progress in...
The isolation of human embryonic stem cells (hESCs) and the discovery of human induced pluripotent stem cell (hiPSC) reprogramming have sparked a renaissance in stem cell biology, in vitro di...
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