Widefield Microscopy as a Screening Tool for 3D Cell Culture

SPONSORED BY: Agilent

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Discover the continued innovation in imaging and microscopy with Agilent's detailed application compendium on widefield microscopy for three-dimensional (3D) cell culture. This resource emphasizes the importance of 3D cell culture in replicating the natural cellular microenvironment, which is crucial for accurate biological research and drug testing. It also highlights the innovative techniques and tools that enhance the creation and analysis of 3D cell cultures, paving the way for more reliable and efficient scientific discoveries.

What you'll learn:

Various imaging assays performed with Agilent BioTek high-content imaging instruments and Gen5 imaging software to characterize phenotypic changes in cancer spheroids in response to different compound treatments.
Optimized spheroid cell culture protocols for common models, including low-adhesion U-bottom plates, Elplasia microwell plates, and the hanging drop method for three common cancer cell lines.
An improved workflow for staining that reduces assay time and minimizes variability, using suitable reagents and liquid handling systems, along with a z-projection algorithm for efficient spheroid image acquisition and automated image analysis in Gen5.

About the Sponsor

Agilent

Agilent (www.agilent.com) is a leader in life sciences, clinical research, diagnostics and applied chemical markets. With over 11,000 employees worldwide, the company provides laboratories worldwide with instruments, services, consumables, applications and expertise, enabling customers to gain the insights they seek. Agilent's expertise and trusted collaboration give them the highest confidence in our solutions. Agilent focuses its expertise on six key markets, where we help our customers achieve their goals: • Food: Agilent products can help ensure that our global food supply is free of contaminants-whether chemical, viral, bacterial or microbiological • Environmental and Forensics: From pesticides to pharmaceutical residues to trace metals, Agilent provides fast, accurate and sensitive analytical methods for monitoring contaminants affecting quality of life • Pharmaceutical: Agilent has one of the broadest solutions portfolios of any company serving the pharmaceutical industry • Diagnostics: Agilent's reliable diagnostic products give doctors the information they need in the fight against cancer and other diseases • Chemical and Energy: The world runs on energy, and energy companies need to locate, extract and refine fuels efficiently and cost-effectively • Clinical Research: Most life sciences and clinical research is done at top-tier universities, with funding from governments around the world