Webinars

JUN 12, 2024 8:00 AM PDT

Mastering Multiplexing: Innovative Tools for Multiplexing in Your qPCR Workflow

Sponsored by: Thermo Fisher Scientific

5 +50pts

Speaker

Event Date & Time

Date: June 12, 2024

Time: 8am PDT, 10am CDT, 11am EDT

Abstract

In quantitative polymerase chain reaction (qPCR), multiplexing increases sample throughout, while reducing the time and cost associated with having separate reactions for each target. Furthermore, the variations that occurs between reactions are minimized when two targets of interest are measured within the same reaction. Due to these benefits, multiplexing in qPCR are widely used in different areas of research, with many applications such as diagnostics, treatment input and response monitoring, etc. While multiplexing in qPCR is advantageous, challenges such as assay design, workflow optimization, and reagent selection need to be addressed to help ensure the accuracy of the experimental output. In this webinar, the factors that impact multiplexing in qPCR are discussed and tools from Thermo Fisher Scientific that are designed for multiplexing success are presented including Applied Biosystems TaqMan Custom QSY and QSY2 based probes and TaqPath DuraPlex 1-Step RT-qPCR Master Mix.

Learning Objectives

Multiplexing for qPCR workflows
- Benefits and condsiderations
- Assay design guidelines
Thermo Fisher Scientific custom oligos for multiplexing needs
- Applied Biosystem TaqMan Custom QSY and QSY2 based probes.
Features of master mixes designed for multiplexing.
- Portfolio of master mixes with multiplexing capabilities

Webinars will be available for unlimited on-demand viewing after live event.

Mastering Multiplexing: Innovative Tools for Multiplexing in Your qPCR Workflow

Chia-An Yen, Ph.D.